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{"sheet":"Keck Oligo Info","index":0,"n_rows":130,"n_cols":4,"rows_kept":130,"cut":false,"hidden":false,"merged":[[0,0,0,4],[2,1,2,4],[9,1,9,4],[30,1,30,4],[13,0,13,4],[15,1,15,4],[17,1,17,4],[19,1,19,4],[21,1,21,4],[22,1,22,4],[23,1,23,4],[26,1,26,4],[27,1,27,4],[28,1,28,4],[29,1,29,4],[55,1,55,4],[32,1,32,4],[33,1,33,4],[34,1,34,4],[35,1,35,4],[46,1,46,4],[47,2,47,4],[49,1,49,4],[50,1,50,4],[51,2,51,4],[54,1,54,4],[70,1,70,4],[56,1,56,4],[57,1,57,4],[60,1,60,4],[4,1,4,4],[61,1,61,4],[62,1,62,4],[63,1,63,4],[64,1,64,4],[67,1,67,4],[68,1,68,4],[69,1,69,4],[89,2,89,4],[71,1,71,4],[72,1,72,4],[73,2,73,4],[74,2,74,4],[77,1,77,4],[78,1,78,4],[82,1,82,4],[83,2,83,4],[84,1,84,4],[85,1,85,4],[88,1,88,4],[79,1,79,4],[103,1,103,4],[90,1,90,4],[91,2,91,4],[92,1,92,4],[95,1,95,4],[96,2,96,4],[97,3,97,4],[98,2,98,4],[99,2,99,4],[100,2,100,4],[101,2,101,4],[102,2,102,4],[118,1,118,4],[104,2,104,4],[105,3,105,4],[106,2,106,4],[107,2,107,4],[108,1,108,4],[109,2,109,4],[110,3,110,4],[111,3,111,4],[112,2,112,4],[116,1,116,4],[117,2,117,4],[129,0,129,4],[119,1,119,4],[120,1,120,4],[121,1,121,4],[125,1,125,4],[126,1,126,4],[127,1,127,4]]}
["Requesting a Quote"]
["Quotes for Unmodified or Modified DNA or RNA"]
["•","Fill out the second worksheet in this file; \"Oligo Quote Request Form\". "]
[null,"◦","For quotes for more than 20 oligos, please duplicate the worksheet and indicate using the pull-down menu whether you would like the worksheets combined into a single quote or not."]
["•","Not all synthesis scales or purification methods are available for each type of oligo.\nPlease choose from the dropdown menus under Synthesis Scale and Purification Method on the next worksheet to ensure your choice is available."]
["•","Return the completed form(s) via email to:"]
[null,null," oligos@yale.edu"]
[]
["Quotes for 96-well Format Plate Orders of ≥48 DNA Oligos"]
["•","Fill out the third worksheet in this file; \"Plate Order Quote Request Form\". "]
["•","Return the completed form(s) via email to:"]
[null,null," oligos@yale.edu"]
[]
["Keck Oligo Synthesis\nFAQs"]
["Ordering Oligos"]
["•","Set up an account with us by clicking the \"Get Password\" link in the upper left of the Keck Oligo Synthesis Ordering webpage at: "]
[null,null,"Keck Oligo Synthesis Ordering Site"]
["•","Once you have received your password, place your order by clicking the \"Order Normal DNA, RNA, or 2'-O methyl RNA\" or \"Order Modified Oligos\" link in the upper left of the Keck Oligo Synthesis Ordering webpage."]
["Bulk Orders (≥24 oligos)"]
["•","Orders of 24 or more oligos can be submitted in an Excel file via email to:"]
[null,null," oligos@yale.edu"]
["•","In the Subject line of the email, please let us know that it is a large or bulk order."]
["•","In the body of the email please give your password, the charging instructions (including any splitting of costs), the synthesis scale and format (individual tubes or a 96-well plate)."]
["•","In the Excel file list the oligo names in the first column and the sequences in the second column."]
[]
["96-well Format Plate Orders (≥48 DNA oligos)"]
["•","Orders for ≥48 oligos supplied to the customer in a 96-well plate (Plate Order) receive a 10% discount."]
["•","Plate orders are available only for unmodified, unpurified DNA oligos."]
["•","Plate orders are available only for 25 nmole (≤66 bases) or 100 nmole (≤119 bases) Synthesis Scales."]
["•","All oligos in a plate order must be the same synthesis scale."]
["•","Plate orders can be submitted in an Excel file via email to:"]
[null,null," oligos@yale.edu"]
["•","In the Subject line of the email, please let us know that it is a Plate order."]
["•","In the body of the email please give your password, the charging instructions (including any splitting of costs), the synthesis scale, and confirm that it is a 96-well plate order."]
["•","In the Excel file list the oligo names in the first column and the sequences in the second column."]
[]
["Order Status"]
["•","The status of your order can be checked by clicking on the \"Check My Orders\" link in the upper left of the Keck Oligo Synthesis Ordering webpage at:"]
[null,null,"Keck Oligo Synthesis Ordering Site"]
[]
["Oligonucleotide Synthesis"]
["•","Nucleic acid syntheses are run on a BioLytic Dr.Oligo-192 or Applied Biosystems 394 synthesizer using beta-cyanoethyl chemistry."]
["•","Unless the researcher request otherwise, normal DNA oligos are delivered in a fully deprotected, lyophillized, unpurified form with the hydroxyl groups at both the 3' and 5' ends."]
["•","Exceptions are Trityl-ON (see below) and some specialty oligos which are supplied on the support without cleavage or deprotection."]
[]
["Synthesis Scales"]
["•","Synthesis scales of 25 nmole, 40 nmole, 200 nmole, and 1.0 µmole are routinely available for unmodified oligos."]
[null,"◦","Synthesis of unmodified oligos at the 40 nmole scale is done on columns that are rated for a 100 nmole synthesis to ensure adequate yields."]
["•","Modified oligos are available at the 40 nmole, 200 nmole, and 1.0 µmole synthesis scales."]
["•","Oligos with a 3' modification can only be made at the 200 nmole or 1 µmole synthesis scales."]
["•","If you need more than 1.0 micromole of a given sequence, please order multiple 1.0 micromole syntheses. "]
[null,"◦","When placing these orders, you must slightly change the name of each oligo name because the database will not accept duplicate sequence names (for obvious reasons): e.g., OLIGO1A, OLIGO1B, OLIGO1C, etc."]
[]
["Sequence Length"]
["•","25 nmole syntheses cannot be longer than 65 bases."]
["•","Sequence length for other synthesis scales cannot exceed 200 residues."]
["•","40 nmole scale syntheses longer than 119 bases must be synthesized on specialty machines at a higher cost per base."]
["•","For sequences longer than 100 bases we recommend synthesis at the 200 nanomole or 1.0 µmole scale. We will synthesize oligos longer than 100mers at 40 nanomole if you specifically request that we do so, but adequate yields cannot be guaranteed."]
[]
["Purification"]
["•","Not all purification methods are available for each synthesis scale. "]
["•","All un-modified oligos synthesized at the 25 or 40 nmole are desalted, free of charge."]
["•","Purification methods available to remove non-full-length oligos are reverse-phase cartridge and HPLC purifications."]
["•","Oligos that will be used in culture can be cartridged-purified non-toxically."]
["•","We do not offer PAGE purification at this time. "]
[]
["Modifications"]
["•","We will incorporate most commercially available modifiers, as long as it is particulate-free and non-radioactive."]
["•","To avoid issues of improper storage and handling, we recommend allowing us to order the modified phosphoramidite/column. "]
["•","To ensure freshness, we order most specialty reagents at the time your order is placed."]
["•","We do not attempt to use leftover reagents as that may result in less than optimal coupling."]
["•","If you provide us with a commercially available phosphoramidite/column, our standard pricing will apply."]
["•","If you provide us with a phosphoramidite/column that is not commercially available, a surcharge for each different modification will apply. "]
[null,"◦","This surcharge covers the additional labor costs of modified phosphoramidite/column handling and storage, synthesizer programming, oligo processing, and e-mail correspondence. "]
[null,"◦","Additionally, since we are not supplying the modified phosphoramidite/column, our usual quality guarantee does not apply."]
[]
["RNA and 2'-O-methyl RNA"]
["•","RNA and 2'-O-methyl RNA are available only at the 1.0 µmole scale."]
["•","RNA oligos are provided with the 2' protecting group removed and cartridge-purified."]
["•","2'-O-methyl RNAs are available unpurified or cartridge purified."]
[]
["Synthesized Oligo Information"]
["•","Each order of individual vials containing dried unmodified oligo is returned to you with associated information sheets that have the sequence, sequence name, total µg, total nmoles, and the µL volume required to reconstitute to 100 µM for each oligo. "]
[null,"◦","Each vial is identified with the sequence name and the sequence."]
["•","Modified oligos will have the total µg, but not the total nmoles or µL volume for reconstitution."]
["•","Plate orders are returned with associated plate positions."]
[]
["Trityl-ON Oligos"]
["•","The 5’-DMT (trityl) protecting group can be left on the oligo if you would like to use this hydrophobic “handle” for purification. Unless you select the “Trityl-On” option, all oligos are supplied “Trityl-Off” with a 5’-OH group."]
[null,"◦","All Trityl-ON oligos are supplied on their synthesis supports without cleavage or deprotection."]
["•","Protocols for cleavage, deprotection and Glen-Pak purification of normal and modified trityl-ON oligos are posted on our website at https://medicine.yale.edu/keck/oligo/services/protocols/. Vials, caps, and non-luer-lock syringes will be supplied with each order."]
[null,"◦","The protocols for deprotection must be followed exactly, and the OD260 from each synthesis should be measured after deprotection to check the unpurified yield of the oligo. The unpurified yield expected from a 1.0 micromole synthesis is about 5 OD260 per residue; yields for the smaller scales are correspondingly less."]
["•","We strongly recommend that, before purification, you also set aside a 1 OD260 aliquot of each synthesis for analysis if you have problems with the oligo(s)."]
[]
["Yields"]
["•","The yield of full-length oligo is affected by factors that include:"]
[null,"◦","Coupling efficiency (ours is estimated to be 99.3% per addition)"]
[null,null,null,"A coupling efficiency of 99.3% means that for each residue addition, 0.7% of the additions fail to couple and the oligo chain is terminated."]
[null,"◦","Oligonucleotide length (the longer the oligo, the fewer full-length oligos there are)"]
[null,"◦","Deprotection and Purification methods"]
[null,"◦","Number and type of modifications (some modified residues do not couple as efficiently as unmodified bases or may sterically hinder the addition of subsequent residues)."]
[null,"◦","3’ residue (specialty synthesis columns do not couple as efficiently as normal bases, so one starts at a disadvantage)"]
[null,"◦","Oligonucleotide sequence (some bases couple less efficiently than others)."]
["•","Factors we can’t always foresee/control include:"]
[null,"◦","The actual loading of the 3’ residue on the synthesis column support. "]
[null,null,null,"The columns we use are loaded for the synthesis scale that is requested (eg 200 nmole dG synthesis column for an oligo with a 3’ G to be made at the 200 nmole synthesis scale), but there is variability from column to column."]
[null,"◦","Variability in the capacity of the purification cartridge. The GlenPak cartridges we use have excess capacity, on average, for the synthesis scales that we make."]
[null,"◦","Conformational changes or modifications that interfere with either synthesis, OD measurements, Q/C procedures, or purification."]
["•","Basic Yield Calculation"]
[null,"◦","To calculate the expected % yield of full-length oligo for an oligo of X length use this formula: % Yield = 0.993^X (0.993 raised to the X power)."]
[null,null,null,"For example, the percent yield of full-length 100-residue oligo is approximately 49.5% (0.993^100) of the synthesis scale."]
[null,null,null,"Therefore, the expected yield of full-length unpurified 100-mer oligo from a 200 nmole synthesis scale would be on the order of 100 nmoles."]
[null,"◦","Assume that some full-length oligo will be lost during purification."]
[]
["Turnaround Times"]
["•"," Orders submitted after 5:00 PM may not be processed until the next business day."]
["•","Turnaround time for normal, unpurified, 25 and 40 nanomole DNA oligos is typically 24 hours for <120-mers."]
[null,"◦","Orders of this type placed before 5pm are usually delivered the following business afternoon via Yale couriers or shipped by FedEx Priority."]
["•","Normal, unpurified DNA oligos >119 bases, normal, unpurified RNA oligos, or those synthesized at the 200 nanomole or 1.0 micromole scale may take 5-7 business days due to longer synthesis and deprotection times."]
["•","Turnaround times for specialty oligos vary but are typically 5-10 business days."]
["•","Cartridge Purification/desalting does not typically change turnaround times."]
["•","HPLC purification typically adds 3-5 business days depending on the size of the order"]
["Note: Inclement weather can sometimes increase turnaround times."]
[]
["Delivery and Shipment"]
["•","Completed Yale orders will be delivered by courier to your P.I.'s delivery location. Deliveries will be made once a day in the mid-afternoon. If no delivery location for your lab has been specified, we will email you when your order is ready and you can pick it up at 300 George St. (room 2127). "]
["•","Non-Yale orders are shipped via Federal Express Priority 1."]
["•","In all cases, the current status for each oligo can be monitored by clicking the \"Check My Orders\" link in the upper left of the Oligo Synthesis Ordering webpage."]
[]
["The Keck Oligo Synthesis Section warrants that its products shall conform to the description provided on the product’s accompanying literature or analytical data. ALL PRODUCT CLAIMS, REGARDLESS OF THEIR NATURE, MUST BE BROUGHT TO OUR ATTENTION WITHIN 60 DAYS OF DELIVERY. Due to the highly complex, customized and experimental nature of some modified oligos, we cannot guarantee the purity and yield of all modified products. Although we will do our best to ensure that the highest quality product is achieved, THE KECK OLIGO SECTION SHALL NOT IN ANY EVENT BE LIABLE FOR INCIDENTAL, CONSEQUENTIAL OR SPECIAL DAMAGES OF ANY KIND RESULTING FROM ANY USE OR FAILURE OF THE PRODUCTS NOR SHALL ITS LIABILITY IN ANY CASE EXCEED THE PURCHASE PRICE OF THE PRODUCT."]

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["Name:"]
["Dept:",{"$nulls":6},">65 Residues Synthesis Scale",null,"Synthesis Scale",null,null," ","25 nmole","40 nmole","200 nmole","1.0 µmole","RNA","2'OMe RNA"]
["Institution:",{"$nulls":6},"40 nmole",null,"25 nmole",null,null,null,"Purification Not Available","Cartridge","Cartridge","Cartridge","2' Deprotection/Cartridge","Cartridge"]
["Address:",{"$nulls":6},"200 nmole",null,"40 nmole",{"$nulls":4},"HPLC","HPLC","HPLC",null,"HPLC"]
["Country:",{"$nulls":6},"1.0 µmole",null,"200 nmole"]
["Email:",{"$nulls":6},"RNA",null,"1.0 µmole"]
[{"$nulls":7},"2'OMe RNA",null,"RNA"]
["If you would like a quote for more than 20 oligos, please duplicate this worksheet and use the pull-down menu in the cell below to indicate if you would like them combined into a single quote or not.",{"$nulls":8},"2'OMe RNA"]
[null,null,"Combine Quotes."]
["Number of Syntheses","Sequence Name","Sequence (5' -> 3')","Synthesis Scale","Purification Method","Modification Details"]

{"sheet":"Plate Order Quote Request Form","index":2,"n_rows":114,"n_cols":3,"rows_kept":114,"cut":false,"hidden":false,"merged":[[0,1,0,2],[1,1,1,2],[2,1,2,2],[3,1,3,2],[4,1,4,2],[11,1,11,2],[12,1,12,2],[13,1,13,2],[14,1,14,2],[5,1,5,2],[7,0,8,2],[10,0,10,2]]}
["Name:"]
["Dept:"]
["Institution:"]
["Address:"]
["Country:"]
["Email:"]
[]
["This Request Form is for Plate Order Quotes only!"]
[]
[]
["FAQs"]
["•","Orders for >47 oligos supplied to the customer in a 96-well plate (Plate Order) receive a 10% discount."]
["•","Plate orders are available only for unmodified, unpurified DNA oligos."]
["•","Plate orders are available only for 25 nmole (≤66 bases) or 40 nmole (≤119 bases) Synthesis Scales."]
["•","All oligos in a plate order must be the same synthesis scale."]
[null,"Synthesis Scale"]
[]
["Oligo Number","Sequence Name","Sequence (5' -> 3')"]
[1]
[2]
[3]
[4]
[5]
[6]
[7]
[8]
[9]
[10]
[11]
[12]
[13]
[14]
[15]
[16]
[17]
[18]
[19]
[20]
[21]
[22]
[23]
[24]
[25]
[26]
[27]
[28]
[29]
[30]
[31]
[32]
[33]
[34]
[35]
[36]
[37]
[38]
[39]
[40]
[41]
[42]
[43]
[44]
[45]
[46]
[47]
[48]
[49]
[50]
[51]
[52]
[53]
[54]
[55]
[56]
[57]
[58]
[59]
[60]
[61]
[62]
[63]
[64]
[65]
[66]
[67]
[68]
[69]
[70]
[71]
[72]
[73]
[74]
[75]
[76]
[77]
[78]
[79]
[80]
[81]
[82]
[83]
[84]
[85]
[86]
[87]
[88]
[89]
[90]
[91]
[92]
[93]
[94]
[95]
[96]